ABSTRACT
@#Considering the increased use of electronic devices, the incidence of dry eye disease(DED)has been rising. The condition has seriously affected people's work and lives. DED, the most common ocular surface disease, can be caused by many factors. The meibomian gland dysfunction(MGD)is one of the main factors. Either abnormal secretions from the meibomian gland or an obstruction of the gland ducts can lead to evaporative dry eye. By summarising the relevant literature, this review addresses the aetiology, pathology, diagnosis and treatment of DED associated with MGD.
ABSTRACT
Objective To explore the relationship between methylenetetrahydrofolate reductase(MTHFR)gene C677T position polymorphism and intracerebral hemorrhage(ICH)in southern Chinese Han population.Methods The MTHFR gene polymorphism were detected in 124 ICH patients(ICH group)and 149 control subjects(normal control group)who were southern Chinese Han population by SNaPshot technique.Results Compared with normal control group,the frequencies of T allele in ICH group and nonlobar ICH subgroup were significantly higher(all P<0.05);and there was no statistical significance of frequencies of C allele in ICH group and nonlobar ICH subgroup, and frequencies of T, C allele in lobar ICH subgroup(all P>0.05).There was no statistical significance of genotype frequency in ICH group, nonlobar ICH subgroup, lobar ICH subgroup and normal control group. Unconditional Logistic regression showed MTHFR gene C677T position polymorphism had no correlation with ICH group and its subgroup(all P>0.05).After confounding factors correction, ICH and nonlobar ICH subtype were significantly associated with T alleles(OR=3.77,95%CI:1.40-10.16,P=0.01;OR=4.19,95%CI:1.30-13.46,P=0.02),and lobar ICH subtype had no correlation with T allele(OR=2.31,95%CI:0.37-14.49,P=0.37).Conclusion MTHFR gene C677T position mutation T allele may be an important risk factor for ICH in the southern Chinese Han population.
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of human alpha-mannosidase Man2c1 transgene on tumor growth and metastasis in mice.</p><p><b>METHODS</b>Hepatoma cell H22 or squamous epithelial carcinoma cell S180 was subcutaneously inoculated into the right armpit of mice (wild type mice and 28#, 35#, and 54# transgenic mice). Tumor size was measured every week. Mice were sacrificed on day 9 or 10 and then the tumors were exercised and weighted. Tumors and lungs were fixed in formaldehyde and sectioned. The sections were stained with hematoxylin/eosin and examined under microscope. The red blood cells in spleen were destroyed by Tris-NH4Cl. Natural killer (NK) cell activity was detected with Yac-1 cell as target.</p><p><b>RESULTS</b>H22 and S180 tumors grew faster in all the three transgenic mice (28#, 35#, and 54#) than in wild type mice. The average size and weight of tumors between the transgenic mice and wild type mice were significantly different (P<0.05). Most tumors in the transgenic mice invaded the surrounding tissues. In contrast, nearly all the tumors in wild type mice were capsulized. Three of 10 28# transgenic mice, 5 of 10 35# transgenic mice, 3 of 10 54# transgenic mice, and 1 of 10 wild type mice showed lung metastasis of H22 tumor. Two of 6 28# transgenic mice, 3 of 6 35# transgenic mice, 1 of 6 54# transgenic mice, and 0 of 6 wild type mice showed lung metastasis of S180 tumor. No difference of NK activity in spleen cells was observed between the transgenic mice and wild type mice.</p><p><b>CONCLUSIONS</b>hMan2c1 transgene promotes growth, invasion, and metastasis of transplanted H22 and S180 tumors in mice. hMan2cl transgene does not affect NK activity in splenocytes.</p>